利用基因芯片分析鸡法氏囊差异表达基因

doi:10.11843/j.issn.0366-6964.2014.07.019

Abstract

Abstract:

To investigate the dynamic changes of gene expression in chicken bursa，the total mRNA extracted from SPF chicken bursa at 18 embryo-days，10 and 30 days-old were hybridized using Agilent Chicken Genome Array.The differentially expressed genes were analyzed with gene ontology (GO) and KEGG to screen the significant signaling pathway.Several differentially expressed genes were verified with real time quantitative PCR.The results showed that the gene detection rate were 79%-85%，and 5 043，2 368 and 1 874 differential expression genes were identified when comparing chicken of 30 days-old with embryo of 18 embryos-days，chicken of 10 days-old compared with embryo of 18 embryos-days，and chicken of 30 days-old compared with chicken of 10 days-old，respectively.Differentially expressed genes were involved in binding functions，cell component，metabolic progress，cell progress，biological regulation，etc.Additionally，cytokine signaling pathway，Toll-like receptor signaling pathway，NOD-like receptor signaling pathway，and mTOR signaling pathway were also included.Gene expression profile of chicken bursa of Fabricius was successfully analyzed，and the function of differentially expressed genes need further study to understand the molecular mechanism of the development in chicken bursa of Fabricius.

CLC Number:

S831.2

HANG Bo-lin， SANG Jian-jun， QIAN Kun， YE Jian-qiang， SHAO Hong-xia，JIN Wen-jie， MEI Mei， MIAO Ji， QIN Ai-jian. Analysis of Differential Expression Genes in Chicken Bursa of Fabricius with Gene Expression Array[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1162-1169.

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Analysis of Differential Expression Genes in Chicken Bursa of Fabricius with Gene Expression Array

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